ChemFH: an integrated tool for screening frequent false positives in chemical biology and drug discovery

High-throughput screening rapidly tests extensive arrays of chemical compounds to identify hit compounds for specific biological targets in drug discovery. However, false-positive results disrupt hit-to-lead progression and waste resources. ChemFH is an integrated online tool that systematically screens for frequent false positives by incorporating large-scale structural alerts, aggregation predictors, and interference filters covering pan-assay interference compounds (PAINS), reactive metabolites, and colloidal aggregators, enabling researchers to flag problematic compounds early in the discovery pipeline.